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Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum

Valeria Villanova, Antonio Emidio Fortunato, Dipali Singh, Davide Dal Bo, Melissa Conte, Toshihiro Obata, Juliette Jouhet, Alisdair R. Fernie, Eric Marechal, Angela Falciatore, Julien Pagliardini, Adeline Le Monnier, Mark Poolman, Gilles Curien, Dimitris Petroutsos, Giovanni Finazzi
Published 17 July 2017.DOI: 10.1098/rstb.2016.0404
Valeria Villanova
Fermentalg SA, 33500 Libourne, FranceLaboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes (UGA), Centre National de la Recherche Scientifique (CNRS), Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Institut National Recherche Agronomique (INRA), Institut de Biosciences et Biotechnologies de Grenoble (BIG), CEA Grenoble, F-38000 Grenoble, France
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Antonio Emidio Fortunato
Laboratoire de Biologie Computationnelle et Quantitative, Sorbonne Universités, UPMC, Institut de Biologie Paris-Seine, CNRS, 15 rue de l'Ecole de Médecine, Paris 75006, France
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Dipali Singh
Department of Biological and Medical Sciences, Oxford Brookes University, Oxford OX3 0BP, UK
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Davide Dal Bo
Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes (UGA), Centre National de la Recherche Scientifique (CNRS), Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Institut National Recherche Agronomique (INRA), Institut de Biosciences et Biotechnologies de Grenoble (BIG), CEA Grenoble, F-38000 Grenoble, France
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Melissa Conte
Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes (UGA), Centre National de la Recherche Scientifique (CNRS), Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Institut National Recherche Agronomique (INRA), Institut de Biosciences et Biotechnologies de Grenoble (BIG), CEA Grenoble, F-38000 Grenoble, France
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Toshihiro Obata
Max-Planck Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476 Golm-Potsdam, Germany
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Juliette Jouhet
Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes (UGA), Centre National de la Recherche Scientifique (CNRS), Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Institut National Recherche Agronomique (INRA), Institut de Biosciences et Biotechnologies de Grenoble (BIG), CEA Grenoble, F-38000 Grenoble, France
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Alisdair R. Fernie
Max-Planck Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476 Golm-Potsdam, Germany
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Eric Marechal
Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes (UGA), Centre National de la Recherche Scientifique (CNRS), Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Institut National Recherche Agronomique (INRA), Institut de Biosciences et Biotechnologies de Grenoble (BIG), CEA Grenoble, F-38000 Grenoble, France
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Angela Falciatore
Laboratoire de Biologie Computationnelle et Quantitative, Sorbonne Universités, UPMC, Institut de Biologie Paris-Seine, CNRS, 15 rue de l'Ecole de Médecine, Paris 75006, France
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Julien Pagliardini
Fermentalg SA, 33500 Libourne, France
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Adeline Le Monnier
Fermentalg SA, 33500 Libourne, France
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Mark Poolman
Department of Biological and Medical Sciences, Oxford Brookes University, Oxford OX3 0BP, UK
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Gilles Curien
Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes (UGA), Centre National de la Recherche Scientifique (CNRS), Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Institut National Recherche Agronomique (INRA), Institut de Biosciences et Biotechnologies de Grenoble (BIG), CEA Grenoble, F-38000 Grenoble, France
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Dimitris Petroutsos
Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes (UGA), Centre National de la Recherche Scientifique (CNRS), Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Institut National Recherche Agronomique (INRA), Institut de Biosciences et Biotechnologies de Grenoble (BIG), CEA Grenoble, F-38000 Grenoble, France
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  • For correspondence: dimitris.petroutsos@cea.fr
Giovanni Finazzi
Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes (UGA), Centre National de la Recherche Scientifique (CNRS), Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Institut National Recherche Agronomique (INRA), Institut de Biosciences et Biotechnologies de Grenoble (BIG), CEA Grenoble, F-38000 Grenoble, France
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Abstract

Diatoms are prominent marine microalgae, interesting not only from an ecological point of view, but also for their possible use in biotechnology applications. They can be cultivated in phototrophic conditions, using sunlight as the sole energy source. Some diatoms, however, can also grow in a mixotrophic mode, wherein both light and external reduced carbon contribute to biomass accumulation. In this study, we investigated the consequences of mixotrophy on the growth and metabolism of the pennate diatom Phaeodactylum tricornutum, using glycerol as the source of reduced carbon. Transcriptomics, metabolomics, metabolic modelling and physiological data combine to indicate that glycerol affects the central-carbon, carbon-storage and lipid metabolism of the diatom. In particular, provision of glycerol mimics typical responses of nitrogen limitation on lipid metabolism at the level of triacylglycerol accumulation and fatty acid composition. The presence of glycerol, despite provoking features reminiscent of nutrient limitation, neither diminishes photosynthetic activity nor cell growth, revealing essential aspects of the metabolic flexibility of these microalgae and suggesting possible biotechnological applications of mixotrophy.

This article is part of the themed issue ‘The peculiar carbon metabolism in diatoms'.

Footnotes

  • One contribution of 16 to a theme issue ‘The peculiar carbon metabolism in diatoms’.

  • Electronic supplementary material is available online at https://dx.doi.org/10.6084/m9.figshare.c.3799462.

  • Accepted April 12, 2017.
  • © 2017 The Author(s)
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5 September 2017
Volume 372, issue 1728
Philosophical Transactions of the Royal Society B: Biological Sciences: 372 (1728)
  • Table of Contents
Theme issue ‘The peculiar carbon metabolism in diatoms’ compiled and edited by Benoît Schoefs, Hanhua Hu and Peter G. Kroth

Keywords

mixotrophy
metabolism
marine diatoms
omics analyses
photosynthesis
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Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum
Valeria Villanova, Antonio Emidio Fortunato, Dipali Singh, Davide Dal Bo, Melissa Conte, Toshihiro Obata, Juliette Jouhet, Alisdair R. Fernie, Eric Marechal, Angela Falciatore, Julien Pagliardini, Adeline Le Monnier, Mark Poolman, Gilles Curien, Dimitris Petroutsos, Giovanni Finazzi
Phil. Trans. R. Soc. B 2017 372 20160404; DOI: 10.1098/rstb.2016.0404. Published 17 July 2017
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Research article:

Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum

Valeria Villanova, Antonio Emidio Fortunato, Dipali Singh, Davide Dal Bo, Melissa Conte, Toshihiro Obata, Juliette Jouhet, Alisdair R. Fernie, Eric Marechal, Angela Falciatore, Julien Pagliardini, Adeline Le Monnier, Mark Poolman, Gilles Curien, Dimitris Petroutsos, Giovanni Finazzi
Phil. Trans. R. Soc. B 2017 372 20160404; DOI: 10.1098/rstb.2016.0404. Published 17 July 2017

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